PUBLICACIONES Y PROYECTOS CADAmpliando el conocimiento
Biology, ecology and demography of the tropical treehopper Ennya maculicornis (Hemiptera: Membracidae): relationships between female fitness, maternal care and oviposition sites.Ecological Entomology.
LILIANA CACERES-SANCHEZ,1 DANIEL TORRICO-BAZOBERRY,1,2 ROMINA COSSIO,1,3 KENIA REQUE,1 SANDRA AGUILAR,1 HERMANN M. NIEMEYER2 and CARLOS F. PINTO1,2,3
Estado: Aceptado – En prensa
Revista: Wiley Online Library
POPULATION EFFECTS OF ALTERNATIVE HOST-PLANT USE IN A TROPICAL INSECT
Carlos F. Pinto1,2,3*, Daniel Torrico-Bazoberry1,2, Luis Flores-Prado4 & Hermann M. Niemeyer1
Revista: Wiley Online Library
Kin recognition capacity in a subsocial treehopper (Hemiptera: Membracidae).
DANIEL TORRICO-BAZOBERRY, LILIANA CACERES-SANCHEZ, LUIS FLORES-PRADO, HERMANN M. NIEMEYER, and CARLOS F. PINTO
Revista: Wiley Online Library
Highly improved enzymatic peptide synthesis by using biphasic reactors.
A. Bastida (2), R. M. Blanco (1), S. G. Zárate (3), E. García-Junceda (2) y J. M. Guisán (1).
Revista: Biocatalysis and Biotransformation
(1) Instituto de Catálisis y petroleoquímica, C/ Marie Curie, (2), Cantoblanco, Madrid, Universidad Autónoma de Madrid, Camino de Valdelatas s/nEspaña CSIC
(2) Instituto de Química Orgánica General, c/Juan de la cierva 3, 28006-Madrid-España CSIC
(3)Universidad Mayor Real y Pontificia de San Francisco Xavier de Chuquisaca. Sucre-Bolivia
The synthesis of hydrophobic peptide as Leu-enkephalin derivatives (N-acetyl phenyl-l leucinamide) from hydrophobic esters (N-acetyl phenylalanine methyl ester) plus a high excess hydrophilic nucleophile (l-leucinamide) catalyzed by immobilized chymotrypsin (α-CT) was studied. By using biphasic systems, the biocatalyst and the necessary high excess of nucleophile remain in the aqueous phase. The hydrophobic acyl donor in the organic phase is partially partitioned into the aqueous phase allowing the synthesis of the peptide. Then the highly hydrophobic reaction product is completely partitioned to the organic phase and it cannot be hydrolyzed by the biocatalyst. Under these conditions, synthetic yields of 95% with respect to the acyl donor were obtained. The excess of nucleophile does not need to be recovered at the end of the synthesis because it remains “immobilized” in the aqueous phase and it can be re-used as well as the immobilized biocatalyst. In such biphasic systems, in each reaction cycle, the synthesis proceeded according to this interesting mass balance: 50 mM N-acetyl phenylalanine methyl ester plus 47.5 mM l-leucinamide were converted into 47.5 mM peptide derivative, that precipitated in the organic phase as a fluffy solid, and 2.5 mM N-acetyl phenyl acid as side product in the aqueous phase. The immobilized biocatalyst (inside a porous structure) is not in contact with the organic phase. Three consecutive reaction cycles were performed, and 95% of peptide was always obtained.
Prevalence of musculoskeletal pain among school teachers from urban and rural areas in Chuquisaca, Bolivia: a cross-sectional study
María Teresa Solis-Soto1*, Anabel Schön2, Angel Solis-Soto3, Manuel Parra2 and Katja Radon2
Revista: Safety and Health at Work
Diet and current asthma symptoms in school-aged children from Oropeza Province –Chuquisaca, Bolivia
María Teresa Solis-Soto 1, Armando Patiño3, Dennis Nowak4, Katja Radon 4
Revista: Open Journal of Asthma
Background: Several studies have linked dietary intake and asthma symptoms, nevertheless the results are still inconclusive and data from developing country are scarce.
Objective: To assess the association of Food patterns and current asthma symptoms in school-aged children from rural and urban areas of Oropeza Province – Bolivia.
Methods: A total of 1655 children attending the ﬁ fth elementary grade were invited to participate in a cross-sectional study in rural and urban areas of Oropeza province. Current Asthma symptoms were deﬁ ned following the International Study of Asthma and Allergies in Childhood (ISSAC) recommendation (written and video questionnaire). Food patterns were deﬁ ned using the ISAAC environmental questionnaire. The Mediterranean diet (MD) score was calculated based on this information. Crude and adjusted logistic regression models were calculated including age, sex and place of living.
Results: The age range was between 9 – 15 years (Median 11), and 47.9% were male. Current asthma symptoms were reported in a 17% considering the written questionnaire (WQ) and in a 6% considering the video questionnaire (VQ). Higher consumption of nuts [(p=0.01, aOR= 1.6(1.1-2.2)], corn [(p=0.03, aOR= 1.5(1.0-2.2)] and fast food [(p=0.06, aOR= 1.4(1.0-2.0)] showed positive association with current asthma symptoms. Greater adherence to the MD was associated with lower risk of asthma symptoms.
Conclusion: Promotion of healthy food patterns in children is needed in order to avoid the rapid increase of non-communicable diseases in this region.
Factores psicosociales del ambiente laboral en profesores de áreas urbanas y rurales de Chuquisaca, Bolivia
María Teresa Solis-Soto, Anabel Schön, Manuel Parra, Katja Radon
Revista: Revista Salud Pública UNC
Objetivo: Comparar las dimensiones de riesgo psicosocial en profesores de áreas urbanas y rurales de Chuquisaca, Bolivia. Método: Se realizó un estudio transversal en 60 escuelas seleccionadas al azar (27 urbanos, 33 rurales) de Chuquisaca, invitando a participar a 1.062 profesores (Respuesta 58%). Se utilizó la versión breve del cuestionario SUSESO-ISTAS21 para medir riesgos psicosociales, calculando odds ratios y su Intervalo de Confianza de 95% a través de modelos de regresión logística ajustados por edad, sexo, grado académico más alto e ingresos.
Resultados: Los profesores de áreas rurales presentaron porcentajes más alto de riesgo psicosocial en comparación con los del área urbana, principalmente en las dimensiones: exigencias psicológicas (ORa 1,6; IC 95% 1,1-2,5), apoyo social y calidad del liderazgo (2,9; 1,9-4,8) y doble presencia (2,2; 1,5-3,4). Conclusiones: Se encontraron diferencias en riesgos psicosociales, de manera desfavorable en profesores de áreas rurales, lo que podría repercutir en la salud física y mental.
Precarious employment and mental distress in school teachers in Chuquisaca, Bolivia
Solis-Soto MT, Schön A, Parra M, Radon K.
Gonzalez A. Radon K, Solis M
Natural selection in the tropical treehopper Alchisme grossa (Hemiptera: Membracidae) on two sympatric hostplants
Daniel Torrico-Bazoberry, Carlos F. Pinto, Luis Flores-Prado, Francisco E. Fontúrbel & Hermann M. Niemeyer
Editorial: Springer Science+Business Media Dordrecht
Abstract The pronotum is the most distinctive and representative structure in treehoppers. Although several functions have been proposed for this structure, its involvement in ﬁtness has not been formally evaluated. Given the high degree of maternal investment exhibited by the subsocial membracid Alchisme grossa (Hoplophorionini), the pronotum has been suggested as a shield for offspring protection. We performed selection gradient analyses on A. grossa in order to evaluate how natural selection is acting upon two traits associated with this structure considering three different ﬁtness proxies: (1) number of eggs, (2) the ratio between the number of ﬁrststage nymphs and the number of eggs (early survival), and (3) the number of third-stage nymphs (late survival). Since A. grossa feeds, oviposits and mates on two alternative host-plants (Brugmansia suaveolens and Solanum ursinum, both Solanaceae), we also evaluated and compared selection gradients between these host-plants. We found positive
Sequestration of tropane alkaloids fromBrugmansia suaveolens(Solanaceae) by the treehopperAlchisme grossa (Hemiptera: Membracidae)
Carlos F. Pinto, Silvia Salinas, Luis Flores-Prado, Javier Echeverría,
Hermann M. Niemeyer
Treehoppers (Hemiptera: Membracidae) are sap-feeding insects distributed mainly in tropical regions.Alchisme grossais a treehopper that has been reported in the Bolivian Yungas forests using mostlyBrugmansia suaveolens(Solanaceae) as host-plant, where adult females oviposit and take care of their nymphs until they molt to adults. Brugmansiais a subtropical genus producing a variety of tropane alkaloids (TAs). We herein report the sequestration by adult males and females of A. grossa of TAs from B. suaveolens, examining separately the distinct body sections of insects. Puried extracts of A. grossa and B. sua- veolenswere analyzed by gas chromatography/mass spectrometry. TAs in A. grossa were the same as those in its host-plant; furthermore, they were equally distributed between sexes and they were differentially allocated within the body of adult individuals. An ecological role for sequestered TAs is discussed.